FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21811351
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA272000.09.207890594
CACGTCT333600.08.93027994
ACGTCTG305600.08.779798594
ACACGTC353100.08.5169594
GCACACG455600.08.47793294
CACACGT407550.08.37061994
GATCGGA1015550.07.46336894
ATCGGAA934850.07.36372494
TAGATCG390350.07.258802494
AGATCGG1092150.07.004448494
TCGGAAG936750.06.942473494
CGGAAGA896600.06.870775794
AGAGCAC664950.06.82637294
AGCACAC606000.06.777048694
GAAGAGC849650.06.59231694
GAGCACA670450.06.518060794
GAGATCG336500.06.42354394
CAGATCG346400.06.253525394
AAGAGCA819650.06.162853794
AAGATCG227750.06.065837494
Produced by FastQC (version 0.11.5)