FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013774
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACA3700.015.24283524-25
TCGTATG27150.014.88812894
CTCGTAT30050.013.76415994
TATGCCG18450.013.7565394
ACGCAAT78850.013.05431194
TCGCGAT3409.183891E-512.4427043
TGTCGCG3156.748469E-411.93688294
CACGCAA95850.011.76875894
CGCTACG1400.003803878611.74968532-33
ATCGCGA3651.7794862E-411.5904662
CGCAATC66300.011.55541694
ATCTCGT49800.011.42003894
CGACGCG4950.011.39353914-15
ATGCCGT17950.011.25940694
TCTCGTA41850.010.78170194
ATGCGTT6554.2724423E-810.7646812
CGCTTAG3500.001629726510.74319594
CGCGCTT4402.910383E-1110.68166558-59
AATCTCG49750.010.48676194
TTCACGC129350.010.42862394
Produced by FastQC (version 0.11.5)