FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6860145
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGAT42150.011.70851894
TCGTATG12900.010.93054594
CGTGATA31850.010.32996594
CAACGTG61100.010.15413694
AACGTGA51150.010.10781594
CACAACG67550.09.5324794
ACAACGT61750.09.514441594
CTCGTAT14450.08.78225794
CGTATGC11950.08.652966594
GTCACAA107350.08.23125694
TATCTCG21350.08.14543294
CGTCTGA287900.07.542417594
TATGCCG11351.9281288E-107.45395794
CACGTCT336450.07.208408494
CAGTCAC143550.07.105044494
ACGTCTG311550.07.090557694
CACACGT390500.06.94488594
TCCAGTC189000.06.938293594
GAACTCC231800.06.833240594
ACTCCAG219450.06.789439794
Produced by FastQC (version 0.11.5)