FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17706874
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA264200.08.14794194
ACGTCTG295700.07.96345694
CACACGT384150.07.94070594
ACACGTC334300.07.761003594
CACGTCT322500.07.68061794
CACCCGT73450.07.29504994
GCACACG419000.06.910060494
AGAGCAC584300.06.41117594
AGCACAC528000.06.30252494
CGTGAGA48500.06.299576392-93
ATCGGAA785450.06.277289494
TCGGAAG770600.06.24577294
GCGTAGC8300.0062116616.229342
TCACCCG89750.06.12726494
ACCCGTG73950.06.101663694
GATCGGA837700.06.020414494
TAGATCG280450.05.999882794
GAGCACA594750.05.950804794
CGGAAGA737150.05.923454394
TCTGAAC335600.05.91024894
Produced by FastQC (version 0.11.5)