FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16156368
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA342100.08.75170294
CACGTCT418700.08.66603694
ACACGTC444600.08.53120294
ACGTCTG378500.08.22050294
CACACGT505100.08.09558294
GCACACG554700.07.134443894
AGCACAC670150.06.950368494
CTGAACT355250.06.893024494
AGAGCAC750450.06.832964494
TGAACTC328450.06.73996894
TCTGAAC381050.06.734678394
CGGAAGA984650.06.61110594
TCGGAAG1033450.06.421720594
GTCTGAA417750.06.390547894
ATCGGAA1067050.06.378080494
GAACTCC317050.06.374511294
GAGCACA746950.06.355299594
GATCGGA1142900.06.28378594
CAGTCAC210100.06.24143394
AACTCCA307400.06.207666494
Produced by FastQC (version 0.11.5)