FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16156368
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA385650.09.30202394
GTCGTGT427500.08.72134294
CGTCGTG478750.08.46534694
GTGTAGG361300.08.4324494
CGTGTAG366650.08.37351394
TAGGGAA296400.08.0422294
AGCGTCG566550.07.87542894
TGTAGGG342750.07.709124694
GTAGGGA324050.07.68971294
GCGTCGT520150.07.53847894
AGAGCGT691900.07.38639494
GAGCGTC630700.07.380038394
AGAGTGT168150.07.32572694
AAGAGCG753700.07.04897894
CGGAAGA984500.06.666776794
AAAGAGT236600.06.517864794
TCGGAAG1035900.06.413137494
ATCGGAA1067850.06.38856694
GATCGGA1147750.06.279731394
TAGATCG399400.06.16835694
Produced by FastQC (version 0.11.5)