FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17140358
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA66900.012.15413994
GTCGTAG67950.011.96632794
ACGTCGT90750.011.5494894
CACGTCG102200.011.45124494
GTCACGT133150.011.01330594
AGTCACG157600.010.58708494
CGTAGAA62100.09.99048494
TCACGTC130500.09.18404394
CGTCTGA581400.08.53676194
CACGTCT697150.08.19132794
TCTGAAC586650.08.12387394
TCGTAGA72900.08.05910394
ACACGTC743400.08.0231294
CTCGTAT25200.07.833448494
CTGAACT539450.07.623616794
ACGTCTG637250.07.61894294
GCACACG901500.07.575374694
CAGTCAC270850.07.32297994
CACACGT823650.07.269941394
CGTATGC21500.07.21405994
Produced by FastQC (version 0.11.5)