FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725505

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725505
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10593862
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC1954.6901368E-721.688219
ATTGCGC4158.4764906E-1015.854973
GTACGCT2802.5039242E-413.4260338
AATCTCG37300.012.97597794
CGTATGC17000.012.43870894
CGCTACG1901.8887407E-512.37002466-67
CGGTTCG3451.05421095E-412.260524
TCGTATG22000.012.17485794
ACGCGCG1555.907645E-412.12762778-79
CACTATG138350.011.9217494
GCTAGCG2800.00380387711.7496645
CGCGACA3309.988337E-411.3917878
CGCGGAC7451.891749E-1011.3553813
ACTATGC113600.011.25128894
CGTCGTA2600.00455371310.8441047
TGCGCGA3953.686873E-410.71472551
CTATGCA111600.010.65290494
CGGCGTA3550.001835242110.5912485
ACGGTCG3550.001835242110.5912485
ACCGATA4006.5847416E-1010.57300212-13
Produced by FastQC (version 0.11.5)