FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13104993
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG64500.012.67926694
CACGATA68600.011.98998294
AGTCACG117100.011.03774694
GTCACGA100600.010.51209294
TCACGAT88200.010.17816294
CGATAGA59600.09.54216892-93
CGTCTGA411000.08.73687594
ACGTCTG451650.08.53328894
CACGTCT496600.08.23411894
AATCTCG26450.08.1740494
ACACGTC533100.08.05827594
CACACGT589550.07.685302794
GACAATC56050.07.63080594
TGAACTC370350.07.424173494
GCACACG640800.07.415376794
GATAGAC78550.07.359758494
GTCTGAA472750.07.227822394
GAACTCC338900.07.07299394
TCCAGTC261750.06.985017394
TCTGAAC424200.06.98030694
Produced by FastQC (version 0.11.5)