FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13104993
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG444550.08.64805894
TCGTGTA471000.08.52163494
TGTAGGG393300.08.32903694
GTGTAGG427450.08.29033294
GTCGTGT516150.08.18596194
GTAGGGA365800.07.991578694
AGAGTGT193150.07.883810594
CGTCGTG566700.07.81238494
TAGGGAA340750.07.66875494
GAGCGTC743250.07.61337994
AGCGTCG670700.07.525959594
GCGTCGT613950.07.524998794
AAGAGTG238750.07.362310494
AGAGCGT811350.07.14234494
AAGAGCG873350.06.59491692-93
AAAGAGT262150.06.43621494
AGGGAAA440000.06.34482594
GGGAAAG392750.06.246550694
TCGGAAG1175500.06.21718394
GAAGAGC1017300.06.186106794
Produced by FastQC (version 0.11.5)