FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149639
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTCG31750.013.02537594
GTCACGC67600.09.59364594
AGTCACG76850.09.17273194
CGTCTGA266300.09.01780994
CTCGGTA31600.08.62565894
ACGTCTG297200.08.52297694
ACACGTC344650.08.34496494
CACACGT399150.08.07679894
CGCTCGG37950.08.04920394
GCTCGGT39750.07.92116394
CACGTCT325350.07.742215694
CACGCTC74000.07.36677894
CGGTAAT28300.07.306637394
GCACACG428050.07.30093494
TCGGTAA30550.07.229994394
TCTGAAC311300.07.095288394
CTGAACT281850.06.919605794
TCACGCT76350.06.832273594
AGAGCAC596150.06.81097894
ATCGGAA841350.06.66927994
Produced by FastQC (version 0.11.5)