FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14149639
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG287150.08.9694194
CGTCGTG365250.08.6728594
GTCGTGT328800.08.57652994
GTAGGGA250550.08.55386894
TCGTGTA296100.08.33322394
GTGTAGG282050.08.33174694
GAGCGTC487500.07.98266494
TAGGGAA234550.07.89501594
TGTAGGG270400.07.856405794
GCGTCGT397050.07.658632394
AGCGTCG434000.07.48308194
AGAGCGT531400.07.455867394
AAGAGCG591650.07.22089794
ATCGGAA837650.06.660092494
AGAGTGT141250.06.6547894
AGATCGG964600.06.470576394
GATCGGA900600.06.450281694
GAAGAGC745700.06.34053694
TCGGAAG858200.06.270600394
CGGAAGA821700.06.200235494
Produced by FastQC (version 0.11.5)