FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9776329
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16250.011.28076594
AATCTCG30200.09.64966194
AACAACC112400.09.19991294
TCACAAC177000.08.92263994
CTCGTAT20100.08.88617594
GTCACAA194150.08.73971294
ACCAATC69050.08.57697694
ACAACCA126200.08.38012494
ATCTCGT38200.08.3670694
CACAACA166100.08.26305394
AGTCACA241500.08.23285794
TCGTATG18700.08.04332694
CAGTCAC261650.07.81440394
TCTCGTA35150.07.755860394
CGTCTGA590300.07.68390294
ACTCCAG390550.07.678410594
ACGTCTG644100.07.596695494
CAACCAA103650.07.573113494
CCAGTCA326050.07.51070794
GAACTCC448950.07.475281794
Produced by FastQC (version 0.11.5)