FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9776329
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT253700.08.99488994
TGTAGGG552700.07.901577594
AAGAGTG306100.07.89902994
CGTGTAG634000.07.775240494
TAGGGAA468750.07.727260694
AAAGAGT329450.07.63786894
GTAGGGA506300.07.63542894
GTGTAGG595900.07.525336394
TCGTGTA672950.07.478402694
GTCGTGT728200.07.232742394
CGTCGTG790900.07.109630694
GCGTCGT854450.07.09086594
GGGAAAG477200.06.96198694
GAAAGAG431350.06.941581794
GAGTGTA74950.06.90913392-93
AGGGAAA520650.06.875983794
GAGTGTT81700.06.825150594
AGCGTCG921350.06.698051594
GGAAAGA465500.06.563194894
CGGTTAG3950.00382628256.52573890-91
Produced by FastQC (version 0.11.5)