FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7189034
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG61850.015.88272794
CTCGTAT73000.015.51717694
TATGCCG35000.015.04071594
CGTATGC54050.014.6093994
TCTCGTA97650.013.91054394
ATGCCGT36400.013.42920994
ATCTCGT112150.012.90832794
AATCTCG118800.012.66053394
GCCGTCT23600.011.75055894
GCAATCT175150.011.61973694
TATGCAA279900.011.58683194
ACTATGC346150.011.52820994
TGCAATC214100.011.4157794
CAATCTC166250.011.25226494
TGCCGTC36350.010.86158994
ATGCAAT248350.010.78770894
CACTATG397600.010.78119694
CTATGCA310000.010.65889494
GTATGCC61050.010.47060494
ACACTAT438600.010.36278194
Produced by FastQC (version 0.11.5)