FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7189034
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG4103.8198777E-1117.1997994
GATCTCG34750.013.12298694
ATCTCGG38550.011.21964694
TAGATCT64450.010.86873894
GTGTAGA140800.010.01692394
TCGGTGG27900.09.94176794
AGTGTAG94250.09.12820894
CGTCGGG10851.5825208E-109.09924494
AGATCTC67150.08.68143494
AGAGTGT839000.08.65729694
GAGTGTA228150.08.44849194
AAGAGTG932650.08.3626494
AAAGAGT999850.08.068694
GAGTGTT200900.08.0499794
TGTACGC5850.00145940528.03636994
GCGTGTA15850.08.00831690-91
GAAAGAG1149500.07.860680694
GAGTGTC175950.07.855499394
AATTCGC6150.00239433327.64206556
GTGGTCG12551.3485987E-87.492073594
Produced by FastQC (version 0.11.5)