FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12293703
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG5850.016.0690542
TATCGCG3102.3064094E-615.1617443
AATTACG9200.011.2389186
ACGCGAA4851.9282601E-510.6599835
CGTACGC4206.5269007E-410.0709928
TACGTTA4600.00150088519.1954786
CGTTACG2350.0018220619.00184388-89
AGAGTGT258450.08.80458194
AAAGAGT331250.08.68631194
TAACGGT10103.6043275E-88.3765912
TGTAGGG580600.08.26777494
ATGCGCG12001.1514203E-98.2252463
TAGGGAA493150.08.11316794
GTAGGGA531700.08.10853794
AAGAGTG321550.08.08567894
ACGTTAC5250.0049632288.0579411
GTGTAGG628950.08.02838194
TCGTGTA707600.07.946633394
ACGAACC10850.07.79718362-63
TACGTCA8451.5472098E-57.7872153
Produced by FastQC (version 0.11.5)