FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11909308
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32900.013.7147294
AATCTCG58150.012.2050394
CGTATGC24700.011.98823494
TCGTATG29300.011.87068494
CGTAACG1500.006362651510.96639356-57
ATCTCGT57700.010.75250894
TCTCGTA50350.010.64184794
AGTGGTC186700.010.27134994
GGTCAAT130050.010.08336594
CTAACGC6555.352307E-69.3285494
GTCAATC120900.09.21369294
GTCACAG390600.09.10909794
TATGCCG21850.08.81950194
TGGTCAA175450.08.78682794
AGTCACA410750.08.62790694
TCACAGT364250.08.56801494
TCAATCT114850.08.47131794
GTGGTCA212400.08.43105894
TCCAGTC577350.08.31185994
CAGTCAC451800.08.20809694
Produced by FastQC (version 0.11.5)