FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5282447
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT105050.09.21824594
CGTGTAG262850.08.78113494
GTAGGGA214800.08.16303494
TCGTGTA280450.08.14625394
TAGGGAA196800.08.14528794
TGTAGGG230650.07.92817594
GTGTAGG248350.07.55241594
GTCGTGT304300.07.50062292-93
AAGAGTG129950.07.451916794
AGCGTCG384450.07.116397494
GAGTGTA32450.07.098366794
GCGTCGT354500.07.06786294
GGGAAAG207550.07.066574694
AAAGAGT139250.06.937876792-93
CGTCGTG328250.06.83161992-93
GGAAAGA205000.06.787579594
AGTGTCG9709.54545E-56.784742494
GAGTGTT36400.06.715510494
AGGGAAA227550.06.631400694
GAGCGTC417850.06.513812594
Produced by FastQC (version 0.11.5)