FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19950588
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT360150.09.52685494
CGTGTAG315900.09.28421194
TCGTGTA321550.09.16492894
CGTCGTG406650.09.08472694
GTGTAGG312000.09.02365194
GCGTCGT453450.08.92449994
AGCGTCG499650.08.66370894
TGTAGGG298450.08.55142194
GAGCGTC566450.08.36390394
AAGAGCG688700.07.94388594
GTAGGGA284550.07.796388694
AGAGCGT626350.07.73662794
ATCGGAA998900.07.236763594
TAGGGAA263450.07.100598394
AGATCGG1153000.06.89325594
CGGAAGA943300.06.79633294
TCGGAAG992550.06.714812894
GATCGGA1073100.06.548035694
TAGATCG385250.06.502713794
GAAGAGC881500.06.47301294
Produced by FastQC (version 0.11.5)