FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7126453
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG5850.012.04939958-59
GATCTCG28750.011.27962594
CGCCGTA5500.011.10708460-61
GCCGTAT6850.010.97611860-61
CGATCGT1953.2856053E-410.84914312-13
GTGTAGA91300.08.90550994
GAGTGTA133550.08.72752794
CGTATCA10650.08.16252962-63
GCGTGTA9907.665767E-88.07043894
AAGAGTG543300.07.906602494
TCGCCGT8950.07.87586558-59
AGAGTGT484350.07.845201592-93
AGTGTAG66250.07.661631694
GAGTGTC112750.07.62812794
CGGTGTA11101.774115E-77.621368494
GGTCGCC12950.07.62064656-57
CCGTATC8355.456968E-127.59713662-63
ATCTCGG32600.07.49668394
GAGTGTT129250.07.49065994
TACGATA3150.0030361677.459152758-59
Produced by FastQC (version 0.11.5)