FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12770792
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA909.506354E-426.11892394
CGCGAAT2008.1490725E-1025.8505463
TCGTACG2801.4816529E-515.1063388
CGTAACG1259.55104E-515.03905156-57
TTAAGCG6300.014.1741257
CCGATCG1859.390351E-713.97214668-69
GCGCTAA1553.8700466E-513.64451312-13
ATCGTAC5301.8553692E-913.3014377
CGTACGC3651.2508224E-512.8760259
CGTTACG2703.42734E-812.18630228-29
GATCGCG2900.00494097411.3451562
CGCGTAA2950.005612460411.1527784
TTACGCG3000.00636020310.9668973
ATTTCGC10150.010.6498877
TACGTTA5354.937403E-610.5422774
CGCGCTA2251.0600919E-410.4449180-81
ATCGGTA7754.3510227E-910.31276194
CGAACGT3800.00247364729.89769794
CGCGGTT4359.002113E-49.7243434
CGTACGT3153.2664375E-69.69759250-51
Produced by FastQC (version 0.11.5)