FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11499730
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA404200.08.67435794
GTGTAGG367700.08.5256494
GTCGTGT444300.08.1453494
CGTCGTG489400.08.01894794
CGTGTAG383100.07.998899594
TGTAGGG346250.07.94073894
GCGTCGT535550.07.933470794
TAGGGAA292950.07.90948594
GTAGGGA317550.07.725972794
AGCGTCG584650.07.685228394
AGAGTGT168650.07.552274794
GAGCGTC649050.07.530954494
AGAGCGT705050.07.119445394
AAAGAGT217950.07.09469794
TTACGCG3650.00161958717.08141834-35
AAGAGCG766750.06.82851494
AAGAGTG205800.06.600050494
GAAGAGC908650.06.23282994
GGGAAAG350550.06.221037494
TCGGAAG1034950.06.212430594
Produced by FastQC (version 0.11.5)