FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7143720
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG3702.3474058E-810.16160558-59
GATCTCG21250.08.85032194
AAGAGTG349550.08.78338394
GAGTGTA83600.08.77356994
AGAGTGT300950.07.90522394
GAAAGAG455800.07.726191594
AAAGAGT375050.07.65966994
GAGTGTT89700.07.547932694
TAGGGAA542400.07.359460494
AGTGTCG23350.07.04756694
GTAGGGA580150.06.90489894
CGCCGTA3750.0021523496.893220460-61
TCGCCGT6503.3501237E-76.86885458-59
CGTGTAG710400.06.717706794
GTCGTGT803650.06.71634494
AGGGAAA563950.06.619693394
TGTAGGG621400.06.59786294
GGGAAAG518050.06.552748794
GTGTAGG668750.06.51739394
AGTGTAG49800.06.51444994
Produced by FastQC (version 0.11.5)