FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8599596
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC172450.08.61268594
CACACGT194200.08.10793594
CGTCTGA135450.07.946413594
GCACACG216000.07.87715794
ACGTCTG149350.07.615963594
CACGTCT162300.07.182040794
AGAGCAC299750.06.836628494
CTGAACT161400.06.31932894
ATCGGAA406000.06.27462794
AGCACAC271000.06.20909794
CGGAAGA385950.06.17435594
TCGGAAG406900.06.087481594
AAGATCG99700.06.034336694
AGATCGG458400.05.92649594
GTCTGAA189550.05.926370694
GATCGGA433000.05.91593494
GAGCACA294000.05.818951692-93
GAACTCC136000.05.771547394
TCGTATG10600.00203721745.764374794
AAGAGCA364050.05.667847694
Produced by FastQC (version 0.11.5)