FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8599596
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTGT165050.08.88275394
CGTCGTG182250.08.40540594
GCGTCGT199850.08.39407294
TCGTGTA149250.07.776625694
CGTGTAG146400.07.73543194
AGCGTCG217300.07.611871794
TGTAGGG143100.07.58544294
AGAGCGT267300.07.471340794
TAGGGAA131600.07.177095494
GTGTAGG148950.07.129785594
GAGCGTC242300.06.903908792-93
AAGAGCG292100.06.90135494
GTAGGGA134300.06.892849494
ATCGGAA406750.06.238421494
CGGAAGA381950.06.20058494
TCGGAAG400550.06.182475694
AGATCGG458300.05.967352494
GATCGGA433650.05.927294794
AAAGAGT120750.05.83730994
TAGATCG160450.05.76945994
Produced by FastQC (version 0.11.5)