FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19731741
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG46050.010.51263294
TCGTATG29650.08.55998594
CTCGTAT33150.08.36512594
CGTCTGA841450.08.06571494
AGTCACC322000.07.92588794
CTGAACT764950.07.784894
ACGTCTG922950.07.679396694
ACACGTC1068350.07.421735394
TCTGAAC823950.07.381376394
CTCCAGT556150.07.301728794
CACGTCT999700.07.301392694
GTCACCA355500.07.17900394
ATCTCGT63650.07.16270894
CACACGT1183550.07.108375594
AACTCCA628050.07.01960394
GCACACG1271500.06.949369494
GTCTGAA917000.06.847641594
ACTCCAG641800.06.83992194
GTATCTC91250.06.798999394
TGAACTC735000.06.791115394
Produced by FastQC (version 0.11.5)