FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19731741
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA687850.08.50025994
AGAGTGT373850.08.3478894
GTAGGGA742850.07.934177494
GTGTAGG858650.07.871331794
CGTGTAG903650.07.848641494
AAAGAGT489050.07.84228194
TGTAGGG796350.07.59591494
GTCGTGT1039550.07.50078194
TCGTGTA954950.07.49591794
CGTCGTG1134500.07.287303494
AAGAGTG464750.07.109538694
GCGTCGT1223700.07.00960294
AGCGTCG1325550.06.988693794
GAGCGTC1446200.06.756653394
GAAAGAG667050.06.658538394
AGAGCGT1565300.06.608881594
GAGTGTA127100.06.471395594
AAGAGCG1689100.06.361014494
AGGGAAA813500.06.158928494
GGGAAAG760000.06.134844394
Produced by FastQC (version 0.11.5)