FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13167128
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA282300.09.1904494
CACGTCT349750.08.90971894
ACGTCTG314150.08.55789894
ACACGTC370900.08.12286794
GCACACG467100.07.939172794
CACACGT416000.07.807155694
TGAACTC270800.07.220259794
AAGAGCA728800.06.855404494
GTCTGAA346750.06.845151494
AGCACAC562200.06.821933394
GAACTCC262250.06.756689594
CTGAACT285350.06.65444294
TCTGAAC313850.06.649193394
AGAGCAC625450.06.537847594
ATCGGAA886400.06.45310994
GAGCACA619000.06.370587394
CGGAAGA837000.06.339815694
GAAGAGC775850.06.257929394
GATCGGA952300.06.24838994
AATCTCG21152.1100277E-106.22236994
Produced by FastQC (version 0.11.5)