FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13167128
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA319950.09.54772894
GTGTAGG300800.08.70254294
CGTGTAG307000.08.55740894
GTCGTGT352550.08.31826394
GCGTCGT431600.08.28651694
TGTAGGG282500.08.2182194
CGTCGTG392750.07.981388694
GTAGGGA263300.07.835783594
AGCGTCG472950.07.830324694
TAGGGAA242800.07.800548694
AAGAGCG632700.07.7028294
GAGCGTC524200.07.270974694
AGAGCGT573600.07.079024394
AGAGTGT137600.07.035866394
AAGAGTG181000.06.8547994
AAAGAGT188900.06.76714994
ATCGGAA888350.06.448940894
CGGAAGA833350.06.389564594
GAAGAGC774500.06.28647494
AGATCGG1013800.06.16549894
Produced by FastQC (version 0.11.5)