FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9103081
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT150750.010.16129794
ACACGTC128700.09.82115594
ACGTCTG110100.09.68784994
GCACACG175800.09.19450694
CGTCTGA99950.09.02624894
CACGTCT121550.08.388679594
ATCGGAA376400.07.802238594
CGGAAGA356350.07.50281694
AGATCGG437250.07.3504794
AGAGCAC259350.07.3376694
GAGCACA258400.07.291899794
GATCGGA406350.07.215611594
TCGGAAG376600.07.124339694
GAAGAGC332650.07.02033394
TCTGAAC138100.06.941052494
AGCACAC233400.06.91535892-93
TAGATCG142700.06.91487294
CAGATCG131850.06.84242394
AAGAGCA323150.06.47060294
AAGATCG97700.06.44464694
Produced by FastQC (version 0.11.5)