FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636942
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG40100.012.30426294
TCACGTG30100.010.92803894
ACGTGTT24250.010.85142894
GCGATAG2055.220511E-410.31826470-71
GTCACGT31700.010.07999494
CGTGTTC24400.09.82179794
CACGTGT28350.09.03345992-93
CAGTCAC69600.08.70945794
AACTCCA106850.08.57572394
ACACGTC189000.08.40360394
CTGAACT133000.08.26751294
ACGTCTG159900.08.14032894
GTTCTAA19700.08.11004694
CGTCTGA146350.08.02708694
TCTAATC17750.07.942066794
AATCTCG8351.3230927E-57.87865794
CACGTCT174600.07.80484794
GAACTCC117400.07.72502494
ACTCCAG107750.07.58826794
CACACGT209400.07.495151594
Produced by FastQC (version 0.11.5)