FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636942
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA118600.09.11646194
GTCGTGT182600.08.59862194
AGAGTGT62900.08.44597492-93
TGTAGGG136650.08.29068894
CGTGTAG156800.08.27458494
AAAGAGT78950.08.21693994
TCGTGTA166050.08.06843194
GTAGGGA127500.07.963916394
GAGTGTA20200.07.912444694
GTGTAGG147800.07.887881894
CGTCGTG200250.07.72336494
AGCGTCG237850.07.68828794
AAGAGTG74350.07.6827692-93
GGAAAGA127700.07.546508394
AGAGCGT285250.07.448964694
AATCCGT3500.00103679257.385336450-51
GCGTCGT217550.07.3468894
GAGCGTC260500.07.14612594
AAGAGCG310200.07.04683894
GAAAGAG116000.07.010859594
Produced by FastQC (version 0.11.5)