FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15060459
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53150.013.35315994
TATGCCG34550.011.83536294
AATCTCG95850.011.13125394
CTCGTAT59400.010.99863394
ATCTCGT95000.010.538197594
GGTAATC158650.010.457911594
TCTCGTA84750.09.98258894
CGTATGC51100.09.28989694
ATGCCGT36750.09.08053394
AGTCACT524800.08.89335894
GTGGTAA224250.08.57237594
TGGTAAT205600.08.52699694
GGTGGTA255100.08.512294
CAGTCAC599850.08.07841994
ACTGGTG369550.08.038115594
GTAATCT178850.07.910207794
CACTGGT422850.07.736299594
GTCACTG514900.07.731626594
CTCCAGT775250.07.669359794
TCACTGG471550.07.62507494
Produced by FastQC (version 0.11.5)