FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15877380
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTGT325800.08.84547294
GTAGGGA691550.08.50443394
TCGTGTA924600.08.48113194
AAAGAGT427300.08.41666794
TGTAGGG750100.08.41094994
TAGGGAA634950.08.3666394
GTGTAGG814000.08.29357394
CGTGTAG866700.08.15270694
AAGAGTG396800.08.0920994
GTCGTGT1008600.07.709534694
CGTCGTG1102050.07.618891294
AGGGAAA720200.07.36974594
AGCGTCG1293250.07.357658494
GAGTGTA104800.07.1331192-93
GAAAGAG588900.07.025103694
GGGAAAG668500.06.990312694
GCGTCGT1190650.06.96113194
GGAAAGA646600.06.761746494
GAGCGTC1412050.06.73197894
AGAGCGT1523000.06.624321594
Produced by FastQC (version 0.11.5)