FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5461188
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAT2155.2000245E-415.2994782
CGTGTAG131150.08.74648394
GTCGTGT148250.08.34013494
GTGTAGG127050.08.32568194
TCGTGTA136400.08.23750194
GCGTCGT180500.08.1522894
CGTCGTG165100.08.05844694
TGTAGGG118500.08.01391994
GTAGGGA112900.07.45368594
TAGGGAA106000.07.273608794
AGCGTCG196200.07.201105692-93
AGAGCGT235500.07.08678794
AAAGAGT84750.06.767559594
GAGCGTC214300.06.73550192-93
AAGAGCG258050.06.66790194
ATCGGAA357200.06.65964494
AGAGTGT61450.06.502928394
AGATCGG408550.06.282888494
GATCGGA383500.06.117121794
TCGGAAG351200.06.10410994
Produced by FastQC (version 0.11.5)