FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825989
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATC49350.014.94940594
TCGCAAT62550.012.39561394
ATCGCAA80100.012.37830994
TCGTATG20550.012.34790794
AATCTCG40150.011.93784794
AGATCGC106050.011.47626294
GATCGCA90600.011.41053294
CGTATGC19550.010.33553894
CTCGTAT24650.010.29409694
ATCTCGT46000.08.78520894
AGTCACA302050.08.69649794
GCAATCT80950.08.64929794
CGCTAGT2450.00266099638.63407470-71
CTCCAGT464350.08.51063494
CAGTCAC336100.08.34674694
ACTCCAG522400.08.23055894
GTCACAG290050.08.05183694
TCTCGTA40350.08.03557594
AACTCCA545850.07.997491494
CGTCTGA814000.07.931835794
Produced by FastQC (version 0.11.5)