FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11825989
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGAGT428700.09.16820694
AGAGTGT329050.09.05855594
TAGGGAA641500.08.31822794
GTAGGGA700550.08.16067394
AAGAGTG388900.08.13595894
GAGTGTA95250.08.04552294
TGTAGGG759950.07.99917294
GAAAGAG553100.07.87966394
CGTGTAG876650.07.781668794
GTGTAGG820650.07.77415994
TCGTGTA935600.07.758694694
CGCATAC9306.775701E-67.57919658
GGGAAAG639100.07.42256394
CGCGACA4151.04625564E-47.360100722-23
AGGGAAA695050.07.27149994
GTCGTGT1012600.07.2337194
CGTCGTG1101050.07.14792594
GGAAAGA613300.07.121546794
AGCGTCG1286400.06.9439994
GCGTCGT1186750.06.88924994
Produced by FastQC (version 0.11.5)