FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17048936
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA189350.010.25179194
ACACGTC246600.09.73963894
ACGTCTG211800.09.07637394
CACACGT287750.08.77150194
GCACACG323350.08.56164994
CACGTCT233950.08.21703694
GATCGGA744850.07.591223794
ATCGGAA685400.07.48847794
GAGCACA484350.07.36541994
TCGGAAG692400.07.36525394
AGAGCAC484200.07.2706394
TAGATCG269150.07.24717494
AGATCGG802250.07.176973394
AAGATCG207150.07.101897294
CGGAAGA654300.07.032678694
AGCACAC430000.06.809803594
CTAGATC112800.06.583593494
AAGAGCA601050.06.537484694
GAAGAGC620050.06.443283694
CAGATCG234800.06.226045692-93
Produced by FastQC (version 0.11.5)