FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13000034
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG450550.08.96150894
CGTCTGA404900.08.50916994
ACACGTC527900.08.24499294
CACGTCT489050.07.77546194
CACACGT588800.07.48001994
TCTGAAC419250.07.36585594
GCACACG637350.07.271597494
TGAACTC364850.07.201589694
GTCTGAA471950.07.140918794
AACTCCA320350.07.11619694
CTGAACT389100.07.01852494
GAGCACA834500.06.99561994
AGTCACA199200.06.748505694
GAACTCC341500.06.744291394
ACTCCAG309950.06.70288294
AGCACAC753650.06.679603694
CTCCAGT285550.06.53490594
AGAGCAC858850.06.490801394
GTCACAG195000.06.43587594
TCCAGTC272800.06.375118794
Produced by FastQC (version 0.11.5)