FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13000034
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG443450.08.99280494
TGTAGGG391300.08.34705394
GTCGTGT513000.08.2942894
GTGTAGG422050.08.18302394
GTAGGGA362650.08.12780494
TCGTGTA465400.08.11556794
TAGGGAA337250.08.07298994
CGTCGTG562000.07.81292294
GAGCGTC730750.07.66318594
GCGTCGT609450.07.481436794
AGCGTCG661750.07.40709694
AAAGAGT248450.07.393622494
AAGAGTG233300.07.2510894
CTAACGA8802.2324244E-46.9207091
AGAGTGT185600.06.89371692-93
AGAGCGT798000.06.83534394
AAGAGCG858700.06.5158894
GAGTGTA63500.06.42030694
ATCGGAA1204200.06.284693794
GGAAAGA398600.06.23087194
Produced by FastQC (version 0.11.5)