FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5964448
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA124450.09.4026894
CACCCTA27850.08.94330394
CACGTCT152050.08.53041194
ACACGTC160700.08.48065594
ACCCTAA28900.08.45576294
ACGTCTG138100.08.37121894
CTGAACT126100.07.788941494
GCACACG197600.07.562884394
CACACGT177900.07.50234392-93
AGAGCAC275200.07.240435694
AGTCACC61050.07.23584894
GTCACCC48200.07.019929494
TCGGAAG374450.06.87755494
GAGCACA265350.06.83621194
CGGAAGA351850.06.678204594
GTCTGAA156650.06.65993394
GATCGGA416600.06.621648394
TCTGAAC138000.06.60647794
AGCACAC245600.06.524891494
TGAACTC121750.06.52326594
Produced by FastQC (version 0.11.5)