FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5964448
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA141050.09.06393694
GTGTAGG130650.08.99397494
GTCGTGT154550.08.85003594
TAGGGAA106450.08.69844494
CGTGTAG134650.08.65697994
TGTAGGG121250.08.41199594
AGAGCGT246250.07.978496694
GTAGGGA112850.07.87166792-93
CGTCGTG168350.07.845121492-93
GAGCGTC223500.07.802206594
GCGTCGT184200.07.782716894
AGCGTCG200050.07.682989694
TCGGAAG373400.06.961003394
AAGAGCG267950.06.954980492-93
CGGAAGA350900.06.697421694
GATCGGA415750.06.63631394
ATCGGAA387500.06.392340794
AGATCGG446150.06.32108194
GAAGAGC330600.06.241409394
CTATATA11301.02504564E-46.23881
Produced by FastQC (version 0.11.5)