FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16652264
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG66400.012.31613894
TAGATCT108850.010.19006594
CCTATCG8058.094503E-99.9300473
CTATCGG13700.09.610074
GTGTAGA233500.09.46028894
CGTACGT7503.0644333E-79.4063741
CTAACCG4650.00165181849.0993327
GTACGAT5406.575065E-48.7096061
ATCTCGG72200.08.65780594
CGAACGG7101.4856856E-58.60554694
GAGTGTA368250.08.43630294
AGAGTGT1328850.08.42125594
AAGAGTG1498750.08.22550394
GTACGCT5150.0041525818.2191621
TCGCTCG7553.2023243E-58.0949678
AAAGAGT1625600.08.09249894
GAGTGTT326850.07.90874694
AGTGTAG166650.07.868506494
TTAACGC7201.6192044E-47.83864551
TCGCGTA4801.2623277E-67.83325392-93
Produced by FastQC (version 0.11.5)