FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473040
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC3954.1654857E-1016.6576194
CACGCGA2751.2440707E-515.38124794
CTCGTAA950.004027910514.84155526-27
CGAGTAA1603.202238E-614.68695492-93
TTACTTC2000.005648674414.1024582
AAGACTA1000.005662130714.09947734-35
CGAAGTT1050.00781572413.42949256-57
CGCGAGT1801.0825086E-513.05507190-91
TCACGCG2855.462425E-912.36796292-93
ACGCGAG2400.00220709711.74956494
GTAAATC1450.004937652511.34440792-93
GCGAGTA1700.001278587711.05841392-93
ACGTCTG19700.010.49707294
AGTCACG4801.4551915E-1110.28086992-93
CTCCAGT12200.010.01602294
TCTGAAC17250.09.53587794
CGTCTGA17350.09.48091694
CAGTCAC7004.1836756E-119.39965194
CACGTCT22200.09.31496994
ACACGTC23950.08.63433592-93
Produced by FastQC (version 0.11.5)