FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7846747
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC174200.09.20082194
ACGTCTG147000.08.92087194
CACGTCT163800.08.60850594
CGTCTGA132200.08.21298394
ACCTAAG35950.07.97537994
TAAGGTC24000.07.8337494
CACACGT198450.07.60305592-93
GCACACG229850.07.545747394
AGAGCAC309700.07.148255394
CTGAACT141900.07.022210694
TCTGAAC153100.06.999709694
CTAAGGT27900.06.90716894
GATCGGA459100.06.572765494
TCGGAAG428550.06.51486494
GAAGAGC376700.06.45082594
CGGAAGA408850.06.44939994
GAGCACA306450.06.42650494
TGAACTC135450.06.41967694
AAGAGCA354400.06.36601994
AGATCGG488450.06.264425894
Produced by FastQC (version 0.11.5)