FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13961348
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG200250.08.56970694
GTCGTGT223050.08.07313394
GCGTCGT267900.08.02027194
CGTCGTG247650.07.7837994
TCGTGTA206450.07.742105592-93
GTGTAGG206900.07.521649494
GTAGGGA184450.07.4430394
AGCGTCG291600.07.36841894
AGAGCGT359750.07.26640494
TAGGGAA182400.07.16581494
GAGCGTC326100.07.151159394
TGTAGGG196300.06.82526692-93
ATCGGAA546400.06.62560694
AAGAGCG392450.06.49322694
GATCGGA585850.06.25970394
TCGGAAG540150.06.20612894
CGGAAGA517800.06.15620794
AGATCGG621200.05.964037494
CTCGACG5200.0016230915.875293378-79
TAGATCG242750.05.79105394
Produced by FastQC (version 0.11.5)