FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005725605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8995658
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG1900.004027625514.8436776
ACTAATC49900.012.05651794
AATCTCG27900.011.45558194
TCGTCGG5304.416441E-610.640988
GTCACTA84050.010.34538194
ACGCTCG3750.00289185710.02700294
CAGTCAC330300.09.93251794
TCGTATG14750.09.55964394
TACGATC4500.00122662049.4009953
TAAGCGT4600.00149909839.1966266
TCGCGAT4600.00150008639.19596194
CTAATCT60800.09.12201794
GTAACGC4150.00663037239.0676531
AGTCACA227550.08.81989794
CACTAAT68500.08.67987792-93
TTAAGCG5609.48986E-48.3937455
CTCGTAT16800.08.39313994
TCCAGTC317550.08.34794294
TCTCGTA29650.08.2431194
TGAACTC484450.08.14001994
Produced by FastQC (version 0.11.5)