FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343076960
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG1999150.064.614253
CCGAGTA2085200.064.056062
GTAGCTG7522450.063.362946
AGTAGCT7851800.061.7175755
CGGAGGT1955500.060.5756387
CCCGAGT2455400.058.7828561
GCTGGGA15255400.056.205049
GAGTAGC5375500.056.056514
CTCGGGA2056700.053.2628365
TTGGGAG11654450.052.269636
GCGGAGG2346650.051.6474086
TCGGGAG2308050.050.8799326
TAGCTGG9667550.050.588047
GGAGGCT11542200.049.8990069
GGCGGAG3030400.048.3806845
CTACTCG2287650.048.255032
TACTCGG2330100.048.0131953
GGGAGGC15616500.046.3866548
GGAGGTT5828500.046.2753878
AGGCGGA3267850.044.520144
Produced by FastQC (version 0.11.5)