FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005725616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005725616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343076960
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA2048600.066.946982
CGAGTAG2010150.066.281883
GTAGCTG7653850.064.534296
AGTAGCT7924450.063.177725
CCCGAGT2441450.060.962531
CGGAGGT2047800.058.5778057
GCTGGGA15482200.058.547949
GAGTAGC5386800.057.890824
TTGGGAG11829200.053.8809246
CTCGGGA2095350.053.861375
TAGCTGG9757050.052.022487
GGAGGCT11659600.051.505599
TCGGGAG2372650.051.046856
CTACTCG2278550.049.7509352
GCGGAGG2480700.049.682136
TACTCGG2323100.049.4136433
GGGAGGC15914250.047.520198
GGCGGAG3185700.046.508815
GCACTTT9992500.046.4793851
GGAGGTT5983400.045.6041648
Produced by FastQC (version 0.11.5)